Identification of Bacteria – Further Information

Identification of Bacteria by 16S rRNA Gene Sequencing

Sequencing of the 16S rRNA gene is commonly used for identification of bacteria as it is a highly conserved component of the translational machinery of all bacteria and is highly suited as a target gene for universal identification. The sequence obtained from the isolate is compared to a comprehensive public database to provide taxonomic placement.

• TC0827 – Bacterial identification by 16S rRNA
• TC0853 – Full 16S rRNA sequencing for high resolution bacterial identification and taxonomic placement

MultiLocus Sequence Typing (MLST)

MLST is a procedure for unambiguous sub-species discrimination of bacterial isolates of a variety of common species using the sequences of internal fragments of several house-keeping genes.  MLST data can also be used to determine mutation and recombination rates in the bacterial population and may be useful for epidemiological tracing and source attribution.