Overview

A murine class IgG1 monclonal antibody, prepared by fusion with NS-1 myeloma cells. The hybridoma supernatant was concentrated by ultrafiltration and affinity purified using Protein-G. The finished product was standardised to a concentration of 1 mg/ml by BCA assay. The monoclonal was raised against the SF87/6 strain of border disease virus isolated from a pig in the UK. The final product was tested by IPX assay against the SF87/6 virus and found to have a working dilution of at least 1/100.

We offer all of our monoclonal antibodies conjugated to horse radish peroxidase and biotin.

Volume

1mg

Presentation

Freeze-dried in a glass vial.

Storage

Store the freeze-dried preparation at +4°C in the dark. Once reconstituted dispense into appropriately sized aliquots and store in 50% glycerol at -18°C or below in the dark. Once diluted to working strength the antibody should be stored at +4°C and discarded at the end of the working day.

Directions For Use

Reconstitute with 1 ml sterile distilled water. Care should be taken to ensure that the contents of the vial are completely reconstituted. This monoclonal antibody is suitable for indirect labelling techniques for the detection of pestivirus antigens in cell culture and tissue sections. It binds to the gp48 (Erns) glycoprotein of the virus.

For more information see product datasheet available on request.

Reagent Group

Antibodies – Monoclonal (mAb) – Murine origin: Bovine viral diarrhoea (BVD) and Border disease (BD) virus monoclonal antibodies

MAb

WS371

Antigen

Erns

ImmunoglobulinClass

IgG1

Detects

BDV

LotNumber

See product label.

ExpiryDate

See product label.