Overview

A murine monclonal antibody WH211 (class IgG1), prepared by fusion with NS-1 myeloma cells. The hybridoma supernatant was concentrated by ultrafiltration and affinity purified using Protein-G. The finished product was standardised to a concentration of 1 mg/ml by BCA assay. The monoclonal was raised against the Baker A strain of CSF virus from the USA. The final product was tested by IPX assay against CSFV and found to have a working dilution of at least 1/100.

MAb available in conjugated form on request.

Volume

1mg

Presentation

1 mg of antibody freeze-dried in a glass vial.

Storage

Store the freeze-dried preparation at +4°C in the dark. Once reconstituted dispense into appropriately sized aliquots and store in 50% glycerol at -18°C or below in the dark. Once diluted to working strength the antibody should be stored at +4°C and discarded at the end of the working day.

Directions For Use

Reconstitute with 1 ml sterile distilled water. Care should be taken to ensure that the contents of the vial are completely reconstituted. This monoclonal antibody is suitable for indirect labelling techniques for the detection of pestivirus antigens in cell cultures and tissue sections.

Reagent Group

Antibodies – Monoclonal

MAb

WH211

Antigen

E2

ImmunoglobulinClass

IgG1

Detects

CSF/BVDV-2

LotNumber

See product label.

ExpiryDate

See product label.