Overview

A murine class IgG1 monoclonal antibody prepared by fusion with NS-1 myeloma cells. The hybridoma supernatant was concentrated by ultrafiltration and affinity purified using Protein-G. The finished product was standardised to a concentration of 1mg/ml by BCA assay. The monoclonal was raised against the Oregon C24V strain of BVDV from USA. The final product was tested by IPX assay against the C24V virus and found to have a working dilution of at least 1/100.

We offer all of our monoclonal antibodies conjugated to horse radish peroxidase and biotin.

Volume

1mg

Presentation

Freeze-dried in a glass vial

Storage

Store the freeze-dried preparation at +2-8°C in the dark. Once reconstituted dispense into appropriately sized aliquotes and store in 50% glycerol at -18°C or below in the dark. Once diluted to working strength the antibody should be stored at +2-8°C and discarded at the end of the working day.

Directions For Use

Reconstitute with 1ml sterile distilled water. Care should be taken to ensure that the contents of the vial are completely reconstituted. This monoclonal antibody is suitable for indirect labelling techniques for the detection of pestivirus antigens in cell culture and tissue sections. It binds to the highly conserved epitope p80/125 (NS23) protein of the virus.

Reagent Group

Antibodies – Monoclonal (mAb) – Murine origin: Bovine viral diarrhoea (BVD) and Border disease (BD) virus monoclonal antibodies

MAb

WB103

Antigen

NS2-3

ImmunoglobulinClass

IgG1

Detects

All pestivirus

LotNumber

See product label

ExpiryDate

See product label