Overview

Alveolar macrophage cells, prepared from the lung washings of 1-5 week old piglets.

Presentation

1ml of cells in a plastic cryovial, containing 4×107 cells

Storage

Store the cryovial in cardice (below -70ºC) during transport. Upon receipt, store the vial in liquid nitrogen (below -120ºC) until ready for use

Directions For Use

Perform all pipetting operations in a Class II safety cabinet to maintain sterility. Defrost the vial using a 37ºC waterbath and transfer the cells to a centrifuge tube. Slowly add approximately 10ml of RPMI or growth media, pre-warmed to 37ºC. Centrifuge at 200-500g for 5 minutes to pellet the cells. Pour off the supernatant and gently resuspend the cells in the appropriate volume of fresh growth media to obtain the desired seeding rate. Transfer the cells to tissue culture flasks or plates as necessary and incubate overnight at 37ºC + 5% carbon dioxide. The following day, examine the cells microscopically and use as necessary. The cells will remain viable for up to 5 days. They cannot be passaged on.

Reagent Group

Porcine Primary Cells

ExpiryDate

Cells should remain viable in liquid nitrogen for many years

SafetyPrecautions

This product poses no significant safety risk.