Overview

Porcine Lung Macrophage (PLM) cells are primary alveolar macrophages prepared from lung washings of piglets aged 1–5 weeks. The cells are supplied cryopreserved at a density of 4 × 10⁷ cells per 1 ml vial for in vitro veterinary research use only.

Following controlled thawing, the cells are intended for immediate seeding into tissue culture vessels and short term culture under recommended conditions (RPMI supplemented with foetal bovine serum and L glutamine). PLM cells are suitable for virus permissibility and host–pathogen interaction studies. As a primary cell preparation, they cannot be passaged and are intended for use for up to five days post thaw. The product can be shipped internationally under frozen conditions to maintain suitability for use on receipt.

Presentation

1ml of cells in a plastic cryovial, containing 4×107 cells

Storage

Store the cryovial in cardice (below -70ºC) during transport. Upon receipt, store the vial in liquid nitrogen (below -120ºC) until ready for use

Directions For Use

Perform all pipetting operations in a Class II safety cabinet to maintain sterility. Defrost the vial using a 37ºC waterbath and transfer the cells to a centrifuge tube. Slowly add approximately 10ml of RPMI or growth media, pre-warmed to 37ºC. Centrifuge at 200-500g for 5 minutes to pellet the cells. Pour off the supernatant and gently resuspend the cells in the appropriate volume of fresh growth media to obtain the desired seeding rate. Transfer the cells to tissue culture flasks or plates as necessary and incubate overnight at 37ºC + 5% carbon dioxide. The following day, examine the cells microscopically and use as necessary. The cells will remain viable for up to 5 days. They cannot be passaged on.

Reagent Group

Porcine Primary Cells

ExpiryDate

Cells should remain viable in liquid nitrogen for many years

SafetyPrecautions

This product poses no significant safety risk.

Additional Information

Growth media for these cells is RPMI + 10% foetal bovine serum + 1% L-glutamine. Penicillin/Streptomycin may be added to guard against bacterial contamination. Amphotericin may be added to guard against fungal contamination but do not use Nystatin. These cells have been tested for fungal contamination and permissibility to PRRS virus.

For in-vitro veterinary use only.