Brucellosis

APHA is an FAO/OIE/WHO International Reference Laboratory for Brucellosis, which is a highly infectious bacterial disease that mainly affects cattle, sheep, pigs and goats. The organism causing brucellosis primarily infects the reproductive organs and thrives in the uterus of pregnant animals, often leading to abortion late in gestation. The organism can remain undetected for prolonged periods as there are no clinical signs until abortion occurs.

APHA Scientific offers a wide range of high quality antigens, antisera and diagnostic kits.

These reagents are available for sale to the animal health industry and veterinary diagnostic laboratories worldwide.

Antigens

Brucella abortus Milk Ring Test Antigen

Inactivated antigen (Brucella abortus (Strain 99) stained with haematoxylin dye and suspended in phenol saline) for use in the Milk Ring Test to detect antibodies to B.abortus. All batches are adjusted to have a comparable level of sensitivity when tested against dilutions made in milk of the International Standard Serum to B.abortus.

Brucella abortus / melitensis / suis Complement Fixation Test

Inactivated antigen for use in the Serum Agglutination Test to detect antibodies to B.abortus/melitensis/suis. At working strength the antigen is standardised to give 50% agglutination with a 1/650 dilution of the International Standard Serum to B.abortus.

Brucella Rose Bengal Test – Standard and Sensitive

Inactivated antigen for use in the Rose Bengal Test to detect antibodies to all smooth strains of Brucella. Sensitive antigen is recommended for the detection of Brucella antibodies in sheep and goats but distribution in the UK is restricted to authorised laboratories. The standard antigen is standardised to give positive reaction with a 1/45 dilution of the International Standard Serum (OIEISS) The Sensitive antigen is standardised to give a positive reaction with a 1/47.5 dilution of the OIEISS.

Brucella abortus / melitensis / suis Serum Agglutination Test

Inactivated antigen for use in the Complement Fixation Test to detect antibodies to B.abortus/melitensis/suis. Standardised to give 50% fixation at a 1/200 dilution of the International Standard Serum (OIEISS).

Antisera

Negative control serum for Brucella abortus

Pooled bovine serum

Negative control serum for Brucella ovis

Pooled ovine serum

Positive control serum for Brucella melitensis

Compared against the International Standard Serum to B.abortus and used for monitoring diagnostic tests.

Positive control serum for Brucella abortus

Compared against the International Standard Serum to B.abortus and used for monitoring diagnostic tests.

Diagnostic kits

BRUCELISA-M

An indirect ELISA kit for the detection of antibodies to Brucella abortus in bovine milk samples. Each kit contains sufficient reagents, including antigen coated plates, to test up to 160 samples.

COMPELISA-160 and COMPELISA-400

A competition ELISA kit for the detection of circulating antibodies to Brucella infection in the serum of all animal species. COMPELISA-160 contains sufficient reagents and standard sera to test 160 samples. COMPELISA-400 contains sufficient reagents and standard sera to test 400 samples.

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Our news

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Welcome to Our New APHA Scientific Website

We’re excited to share our new and improved APHA Scientific website. This project reflects our commitment to providing a more modern, accessible, and user-friendly platform to our global commercial customer base.

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APHA Scientific at WAVLD 2025: Advancing Veterinary Diagnostics Together

We were pleased to attend the World Association of Veterinary Laboratory Diagnosticians (WAVLD) 2025 conference in Calgary, Canada, as a Silver Sponsor.

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APHA Scientific – Launch of PT-LIMS: Our New Laboratory Information Management System

We’re excited to announce the launch of PT-LIMS, our improved Proficiency Testing Laboratory Information Management System, now live for all registered customers.

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New price Lists Available for 2025–2026 Proficiency Testing Schemes & Biological Reagents

We’re pleased to announce the release of our updated price lists for the 2025–2026 contract year.

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VETQAS Terms and Conditions (T&Cs)

We want to let you know that our VETQAS T&Cs have recently been updated to reflect changes in business practices and legal requirements.

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APHA Scientific – Successful Accreditation Visit and Transition to New ISO Standard

In February, VETQAS welcomed assessors for our annual UKAS accreditation visit, a vital part of maintaining our high standards and commitment to quality.

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VETQAS Proficiency Testing – Help Us Improve with Our Customer Survey

VETQAS Proficiency Testing are always looking for ways to improve our services, and your feedback plays a crucial role in helping us achieve that goal.

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APHA Scientific attending AAVLD 2024

APHA Scientific business development team is exhibiting at the American Association of Veterinary Laboratory Diagnosticians annual meeting which takes place on October 12 – 13, 2024 at Gaylord Opryland Resort & Convention Center in Nashville, USA.

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